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mouse preb cdna orf clones  (Sino Biological)


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    Structured Review

    Sino Biological mouse preb cdna orf clones
    Based on their conserved domain compositions, <t>PREB,</t> CERS2, and NAT8F3 are new candidate MBTRs ( a ) AlphaFold-predicted structures for three potential MBTRs, here showing the mouse proteins. Transmembrane domains are marked in yellow. The domains that comprise the combination with the transmembrane domain are colored blue. ( b ) Phylogenetic trees showing 10 orthologs of each candidate protein with their Pfam domain annotations (top: PREB, middle: CERS2, bottom: NAT8F3 and NAT8F7). The TLC domain includes internal transmembrane domains. The same color scheme as panel ( a ) was applied.
    Mouse Preb Cdna Orf Clones, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+preb+cdna+orf+clones/Mouse+Preb+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-HA+tag/bio_rxiv__64898__2025__12__20__695554-63-0-8
    Average 94 stars, based on 1 article reviews
    mouse preb cdna orf clones - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinations"

    Article Title: Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinations

    Journal: bioRxiv

    doi: 10.64898/2025.12.20.695554

    Based on their conserved domain compositions, PREB, CERS2, and NAT8F3 are new candidate MBTRs ( a ) AlphaFold-predicted structures for three potential MBTRs, here showing the mouse proteins. Transmembrane domains are marked in yellow. The domains that comprise the combination with the transmembrane domain are colored blue. ( b ) Phylogenetic trees showing 10 orthologs of each candidate protein with their Pfam domain annotations (top: PREB, middle: CERS2, bottom: NAT8F3 and NAT8F7). The TLC domain includes internal transmembrane domains. The same color scheme as panel ( a ) was applied.
    Figure Legend Snippet: Based on their conserved domain compositions, PREB, CERS2, and NAT8F3 are new candidate MBTRs ( a ) AlphaFold-predicted structures for three potential MBTRs, here showing the mouse proteins. Transmembrane domains are marked in yellow. The domains that comprise the combination with the transmembrane domain are colored blue. ( b ) Phylogenetic trees showing 10 orthologs of each candidate protein with their Pfam domain annotations (top: PREB, middle: CERS2, bottom: NAT8F3 and NAT8F7). The TLC domain includes internal transmembrane domains. The same color scheme as panel ( a ) was applied.

    Techniques Used:

    PREB without its transmembrane domain undergoes nuclear translocation and binds to the butyrophilin locus. ( a ) Predicted structure of PREB generated by AlphaFold, highlighting the WD repeat domains, the transmembrane domain, and the site of an engineered C-terminal truncation. ( b ) Schematic of the two constructs created to assay the mouse full-length PREB protein and PREBΔTM, lacking the C-terminal transmembrane domain. The N-terminal biotinylation site is indicated. ( c ) Western blot results of two biological replicates of ES cells expressing PREB and PREBΔTM. Two bands for PREB were detected by Streptavidin-HRP, suggesting C-terminal processing of a full-length PREB when expressed in mouse ES cells. ( d ) Immunofluorescence confocal microscopy images of PREB and PREBΔTM following Dox induction. PREB localizes predominantly to a perinuclear region, forming a ring-like shape. PREBΔTM displays diffused nuclear localization. Rightmost panels show zoomed images corresponding to the boxed regions. ( e ) bioChIP-seq profiles for PREB, PREBΔTM, BirA-only Strep-pulldown, and IgG ChIP-seq control samples. PREBΔTM samples show substantially stronger DNA-binding activity than full-length PREB. ( f ) Representative locus-specific binding profiles of PREB and PREBΔTM at the Btn1a1 (butyrophilin subfamily 1 member A1) gene. PREBΔTM shows markedly higher peak intensities, consistent with enhanced nuclear localization in the absence of the transmembrane domain.
    Figure Legend Snippet: PREB without its transmembrane domain undergoes nuclear translocation and binds to the butyrophilin locus. ( a ) Predicted structure of PREB generated by AlphaFold, highlighting the WD repeat domains, the transmembrane domain, and the site of an engineered C-terminal truncation. ( b ) Schematic of the two constructs created to assay the mouse full-length PREB protein and PREBΔTM, lacking the C-terminal transmembrane domain. The N-terminal biotinylation site is indicated. ( c ) Western blot results of two biological replicates of ES cells expressing PREB and PREBΔTM. Two bands for PREB were detected by Streptavidin-HRP, suggesting C-terminal processing of a full-length PREB when expressed in mouse ES cells. ( d ) Immunofluorescence confocal microscopy images of PREB and PREBΔTM following Dox induction. PREB localizes predominantly to a perinuclear region, forming a ring-like shape. PREBΔTM displays diffused nuclear localization. Rightmost panels show zoomed images corresponding to the boxed regions. ( e ) bioChIP-seq profiles for PREB, PREBΔTM, BirA-only Strep-pulldown, and IgG ChIP-seq control samples. PREBΔTM samples show substantially stronger DNA-binding activity than full-length PREB. ( f ) Representative locus-specific binding profiles of PREB and PREBΔTM at the Btn1a1 (butyrophilin subfamily 1 member A1) gene. PREBΔTM shows markedly higher peak intensities, consistent with enhanced nuclear localization in the absence of the transmembrane domain.

    Techniques Used: Translocation Assay, Generated, Construct, Western Blot, Expressing, Immunofluorescence, Confocal Microscopy, ChIP-sequencing, Control, Binding Assay, Activity Assay

    Related Articles

    Clone Assay:

    Article Title: Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinations
    Article Snippet: In order to create the modified construct pSBFB, the luciferase open reading frame (ORF) from the pSBtet-GP vector (Addgene, 60495) was replaced with a FLAG tag, a biotinylation site, and multiple cloning sites containing Notl and NheI restriction sites as previously described ( ). .. Mouse Preb cDNA ORF clones were obtained from Sino Biological (MG5A1071-CY) and amplified using primers designed to produce constructs encoding PREB with or without the transmembrane domain (PREBΔTM) ( Supplementary Table S1 ), with the incorporation of Notl and NheI restriction sites (NEB, R3189 and R3131). .. The amplified products were cloned into Notl/NheI-digested pSBFB vector using T4 DNA ligase (NEB, M0202).

    Amplification:

    Article Title: Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinations
    Article Snippet: In order to create the modified construct pSBFB, the luciferase open reading frame (ORF) from the pSBtet-GP vector (Addgene, 60495) was replaced with a FLAG tag, a biotinylation site, and multiple cloning sites containing Notl and NheI restriction sites as previously described ( ). .. Mouse Preb cDNA ORF clones were obtained from Sino Biological (MG5A1071-CY) and amplified using primers designed to produce constructs encoding PREB with or without the transmembrane domain (PREBΔTM) ( Supplementary Table S1 ), with the incorporation of Notl and NheI restriction sites (NEB, R3189 and R3131). .. The amplified products were cloned into Notl/NheI-digested pSBFB vector using T4 DNA ligase (NEB, M0202).

    Construct:

    Article Title: Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinations
    Article Snippet: In order to create the modified construct pSBFB, the luciferase open reading frame (ORF) from the pSBtet-GP vector (Addgene, 60495) was replaced with a FLAG tag, a biotinylation site, and multiple cloning sites containing Notl and NheI restriction sites as previously described ( ). .. Mouse Preb cDNA ORF clones were obtained from Sino Biological (MG5A1071-CY) and amplified using primers designed to produce constructs encoding PREB with or without the transmembrane domain (PREBΔTM) ( Supplementary Table S1 ), with the incorporation of Notl and NheI restriction sites (NEB, R3189 and R3131). .. The amplified products were cloned into Notl/NheI-digested pSBFB vector using T4 DNA ligase (NEB, M0202).



    Similar Products

    mouse preb cdna orf clones  (Sino Biological)
    94
    Sino Biological mouse preb cdna orf clones
    Based on their conserved domain compositions, <t>PREB,</t> CERS2, and NAT8F3 are new candidate MBTRs ( a ) AlphaFold-predicted structures for three potential MBTRs, here showing the mouse proteins. Transmembrane domains are marked in yellow. The domains that comprise the combination with the transmembrane domain are colored blue. ( b ) Phylogenetic trees showing 10 orthologs of each candidate protein with their Pfam domain annotations (top: PREB, middle: CERS2, bottom: NAT8F3 and NAT8F7). The TLC domain includes internal transmembrane domains. The same color scheme as panel ( a ) was applied.
    Mouse Preb Cdna Orf Clones, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+preb+cdna+orf+clones/Mouse+Preb+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-HA+tag/bio_rxiv__64898__2025__12__20__695554-63-0-8
    Average 94 stars, based on 1 article reviews
    mouse preb cdna orf clones - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Based on their conserved domain compositions, PREB, CERS2, and NAT8F3 are new candidate MBTRs ( a ) AlphaFold-predicted structures for three potential MBTRs, here showing the mouse proteins. Transmembrane domains are marked in yellow. The domains that comprise the combination with the transmembrane domain are colored blue. ( b ) Phylogenetic trees showing 10 orthologs of each candidate protein with their Pfam domain annotations (top: PREB, middle: CERS2, bottom: NAT8F3 and NAT8F7). The TLC domain includes internal transmembrane domains. The same color scheme as panel ( a ) was applied.

    Journal: bioRxiv

    Article Title: Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinations

    doi: 10.64898/2025.12.20.695554

    Figure Lengend Snippet: Based on their conserved domain compositions, PREB, CERS2, and NAT8F3 are new candidate MBTRs ( a ) AlphaFold-predicted structures for three potential MBTRs, here showing the mouse proteins. Transmembrane domains are marked in yellow. The domains that comprise the combination with the transmembrane domain are colored blue. ( b ) Phylogenetic trees showing 10 orthologs of each candidate protein with their Pfam domain annotations (top: PREB, middle: CERS2, bottom: NAT8F3 and NAT8F7). The TLC domain includes internal transmembrane domains. The same color scheme as panel ( a ) was applied.

    Article Snippet: Mouse Preb cDNA ORF clones were obtained from Sino Biological (MG5A1071-CY) and amplified using primers designed to produce constructs encoding PREB with or without the transmembrane domain (PREBΔTM) ( Supplementary Table S1 ), with the incorporation of Notl and NheI restriction sites (NEB, R3189 and R3131).

    Techniques:

    PREB without its transmembrane domain undergoes nuclear translocation and binds to the butyrophilin locus. ( a ) Predicted structure of PREB generated by AlphaFold, highlighting the WD repeat domains, the transmembrane domain, and the site of an engineered C-terminal truncation. ( b ) Schematic of the two constructs created to assay the mouse full-length PREB protein and PREBΔTM, lacking the C-terminal transmembrane domain. The N-terminal biotinylation site is indicated. ( c ) Western blot results of two biological replicates of ES cells expressing PREB and PREBΔTM. Two bands for PREB were detected by Streptavidin-HRP, suggesting C-terminal processing of a full-length PREB when expressed in mouse ES cells. ( d ) Immunofluorescence confocal microscopy images of PREB and PREBΔTM following Dox induction. PREB localizes predominantly to a perinuclear region, forming a ring-like shape. PREBΔTM displays diffused nuclear localization. Rightmost panels show zoomed images corresponding to the boxed regions. ( e ) bioChIP-seq profiles for PREB, PREBΔTM, BirA-only Strep-pulldown, and IgG ChIP-seq control samples. PREBΔTM samples show substantially stronger DNA-binding activity than full-length PREB. ( f ) Representative locus-specific binding profiles of PREB and PREBΔTM at the Btn1a1 (butyrophilin subfamily 1 member A1) gene. PREBΔTM shows markedly higher peak intensities, consistent with enhanced nuclear localization in the absence of the transmembrane domain.

    Journal: bioRxiv

    Article Title: Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinations

    doi: 10.64898/2025.12.20.695554

    Figure Lengend Snippet: PREB without its transmembrane domain undergoes nuclear translocation and binds to the butyrophilin locus. ( a ) Predicted structure of PREB generated by AlphaFold, highlighting the WD repeat domains, the transmembrane domain, and the site of an engineered C-terminal truncation. ( b ) Schematic of the two constructs created to assay the mouse full-length PREB protein and PREBΔTM, lacking the C-terminal transmembrane domain. The N-terminal biotinylation site is indicated. ( c ) Western blot results of two biological replicates of ES cells expressing PREB and PREBΔTM. Two bands for PREB were detected by Streptavidin-HRP, suggesting C-terminal processing of a full-length PREB when expressed in mouse ES cells. ( d ) Immunofluorescence confocal microscopy images of PREB and PREBΔTM following Dox induction. PREB localizes predominantly to a perinuclear region, forming a ring-like shape. PREBΔTM displays diffused nuclear localization. Rightmost panels show zoomed images corresponding to the boxed regions. ( e ) bioChIP-seq profiles for PREB, PREBΔTM, BirA-only Strep-pulldown, and IgG ChIP-seq control samples. PREBΔTM samples show substantially stronger DNA-binding activity than full-length PREB. ( f ) Representative locus-specific binding profiles of PREB and PREBΔTM at the Btn1a1 (butyrophilin subfamily 1 member A1) gene. PREBΔTM shows markedly higher peak intensities, consistent with enhanced nuclear localization in the absence of the transmembrane domain.

    Article Snippet: Mouse Preb cDNA ORF clones were obtained from Sino Biological (MG5A1071-CY) and amplified using primers designed to produce constructs encoding PREB with or without the transmembrane domain (PREBΔTM) ( Supplementary Table S1 ), with the incorporation of Notl and NheI restriction sites (NEB, R3189 and R3131).

    Techniques: Translocation Assay, Generated, Construct, Western Blot, Expressing, Immunofluorescence, Confocal Microscopy, ChIP-sequencing, Control, Binding Assay, Activity Assay